According to a medical study published in the journal nature biotechnology on September 9, a team of us scientists described an improved ultra-low temperature technology that can maintain the human liver at -4℃ and extend its survival time in vitro by 27 hours.
Currently, the supply of organs cannot meet the clinical demand, and one of the fundamental reasons for the shortage of donor organs is that it is difficult for existing technologies to extend the storage time of organs beyond a few hours. Current cryopreservation standards allow the liver to survive for no more than 12 hours at +4℃.
Researcher kirkut jurgen of Massachusetts general hospital and harvard medical school in the United States and colleagues have previously extended the preservation time of rat livers at ultra-low temperatures of -6℃ without freezing. However, there have been many limitations in applying the ultra-cold method to human organs.
This time, the institute’s Rainier devrieth and Sharon taser, along with jurgen’s team, developed an improved ultra-cold technique that prevents the human liver from freezing. The ultra-cryogenic technique, which maintains human livers at -4 ° c, extends donor organ survival by 27 hours compared with existing preservation methods.
In addition, the team used mild hypothermia mechanical perfusion technology, which can continuously pump oxygen and nutrients into the liver at about 20℃ to rewarm the ultra-low temperature liver. The activity of the liver did not change before and after cryogenic treatment, and it withstood stress in the simulated transplant.
The results support the use of sub-zero organ preservation techniques in clinical practice, the researchers concluded. However, it is necessary to first test the long-term survival of cryogenic livers after transplantation in large animal models.